A high-resolution method for amino acid analysis of physiological fluids containing mixed disulfides.
Open Access
- 1 April 1988
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 34 (4) , 719-723
- https://doi.org/10.1093/clinchem/34.4.719
Abstract
In this paper we report a high-resolution method and special buffers for the analysis for 45 common amino acids and related compounds and seven less-common components occasionally found in physiological fluids. Careful optimization of the chromatographic protocol resolves cysteine-penicillamine mixed disulfide, penicillamine disulfide, and dihydroxyphenylalanine from cystine and methionine; cysteine-homocysteine mixed disulfide is eluted between tyrosine and phenylalanine. Argininosuccinic acid is eluted before ethanolamine; glycerophosphoethanolamine is separated from taurine; glucosaminic acid is eluted between urea and aspartic acid. A Beckman Instruments System 6300 Amino Acid Analyzer was used. System performance is examined in terms of coefficient of variation (CV) over the concentration range of 1.0-5.0 nmol/50 microL; average CV for all components is better than 1.5%. Method performance is assessed through the analysis of normal and abnormal urine and plasma specimens.This publication has 1 reference indexed in Scilit:
- Automatic amino acid analysis of blood serum and plasmaClinical Biochemistry, 1967