Sporulation de Entomophthora obscura Hall & Dunn en culture liquide

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Abstract
Growth and production of azygospores of E. obscura were studied through batch cultures in media containing glucose or vegetable oil and yeast extract. In media containing 3% glucose and 1% yeast extract, an 8 h lag phase occurred and sporulation began only at the 40th h of culture; spore maturation lasted 4 days in average. Young mycelial stages were characterized by high nucleic acid and protein but low lipid concentrations. During sporogenesis stages, the quantity of lipids and chitin increased and the concentration of total polyosides (chitin excluded) was lower in comparison with the mycelial stages. Sporulation was induced by medium starvation in C and (or) N. The only sporulation inhibitor which allowed considerable growth was sodium fluoroacetate.
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