DETERMINATION OF SPERM MIGRATION RATE IN SMALL SAMPLES OF CERVICAL MUCUS

Publisher Website
Google Scholar
Abstract
A capillary method for the assessment of the migration rate of normal sperm in small samples of human cervical mucus (CXM) has been evaluated. The following results have been obtained: Sperm migrated at a constant rate in the CXM. Measurement of sperm migration after 5 minutes correlated well with measurements made after longer intervals. The coefficient of variation varied between 3.3 and 26.8 %, higher values being obtained in samples with a low sperm penetration. Different sperm samples migrated in the same sample of CXM at a similar rate. Dilution of a sperm sample of good motility to 1 million/ml did not affect the readings. Tests performed at room temperature gave the same results as those performed at 37°C. Capillary size was not of crucial importance. Freezing of a sample to −23°C was possible for one week. Longer periods of storage interfered with sperm penetration. The migration rate of the most advanced sperm did not differ from that of less advanced sperm.

This publication has 0 references indexed in Scilit: