In Vitro Response Characteristics of Brain Tumor Cell Lines to Hyperthermia

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Abstract
Basic aspects of hyperthermia of brain tumor were studied in in vitro experimental systems. Three rat brain tumor cell lines and three human glioblastoma cell lines were used in this study. Heat sensitivity was tested in microplate by water bath hyperthermia. Under 1 hour hyperthermia below 42°C, many of the cell lines grew rapidly, the same as control, though a few lines showed minor suppression. However, all the cell lines showed irreversible cellular damage following 1 hour hyperthermia at 46°C. In the range between 43 and 45°C each cell line demonstrated specific sensitivity. Thermotolerance induced by preheating showed again a markedly varied pattern. Three of them demonstrated apparent thermotolerance, but the others did not. Comparison of sensitivity to heat and ACNU revealed an almost reversed correlation. For example, heat-sensitive cell lines like EB679 and T98 were moderately resistant to ACNU. In contrast ACNU sensitive cell lines like SK-MG1 and AO2 were highly resistant to hyperthermia. These results may indicate a usefulness of the combined modality of treatment with ACNU and hyperthermia, because of their totally different mechanisms.
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