Cloning and characterization of a cDNA encoding the chloroplastic copper/zinc-superoxide dismutase from pea.
- 1 October 1988
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 85 (20) , 7661-7665
- https://doi.org/10.1073/pnas.85.20.7661
Abstract
A cDNA clone encoding the chloroplastic copper/zinc-superoxide dismutase of pea (Pisum sativum L.) was isolated from a cDNA library constructed in .lambda.gt11 from leaf mRNA. Nucleotide sequence analysis of the 875-base-pair clone revealed that it contained the complete coding sequence of the mature superoxide dismutase isozyme subunit, along with sequence information for a 48-amino acid N-terminal transit peptide. The deduced amino acid sequence of the mature subunit proved to be 64-87% homologous with amino acid sequences of copper/zinc-superoxide dismutases from other plant species. In vitro transcription, followed by cell-free translation, of the cDNA resulted in the formation of a 23.5-kDa precursor polypeptide, which, upon incubation with isolated pea chloroplasts, was imported and processed to its mature subunit molecular mass of 17.4 kDa.This publication has 26 references indexed in Scilit:
- In vitro synthesis, importation and processing of Mn-superoxide dismutase (SOD-3) into maize mitochondriaBiochimica et Biophysica Acta (BBA) - General Subjects, 1987
- THE TRANSPORT OF PROTEINS INTO CHLOROPLASTSAnnual Review of Biochemistry, 1986
- Primary Structure of Cu-Zn Superoxide Dismutase ofBrassica oleracea.Proves Homology with Corresponding Enzymes of Animals, Fungi and ProkaryotesBiological Chemistry Hoppe-Seyler, 1986
- Nucleotide sequence analysis of the nuclear gene coding for manganese superoxide dismutase of yeast mitochondria, a gene previously assumed to code for the Rieske iron‐sulphur proteinEuropean Journal of Biochemistry, 1985
- Identification, developmental regulation, and response to heat shock of two antigenically related forms of a major nuclear envelope protein in Drosophila embryos: application of an improved method for affinity purification of antibodies using polypeptides immobilized on nitrocellulose blotsThe Journal of cell biology, 1984
- A novel chromatographic procedure for purification of bacterial plasmidsAnalytical Biochemistry, 1983
- “Western Blotting”: Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein AAnalytical Biochemistry, 1981
- Subcellular Localisation and Identification of Superoxide Dismutase in the Leaves of Higher PlantsEuropean Journal of Biochemistry, 1978
- Superoxide dismutase: Improved assays and an assay applicable to acrylamide gelsAnalytical Biochemistry, 1971
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970