Phage T4-modified RNA polymerase transcribes T4 late genes in vitro.
Open Access
- 1 December 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (12) , 5305-5309
- https://doi.org/10.1073/pnas.74.12.5305
Abstract
Initiation of T4 late RNA synthesis was achieved in an in vitro system prepared from Escherichia coli cells infected with wild-type or maturation-defective mutant T4 phage. The system uses a cellophane membrane as a mechanical support for concentrated cell lysates and for added streptolydigin-resistant RNA polymerases. Transcriptional activity and selectivity of added RNA polymerases are tested while endogenous RNA polymerase activity is inhibited by streptolydigin. T4-modified RNA polymerase is required for substantial stimulation of T4 late RNA synthesis.Keywords
This publication has 18 references indexed in Scilit:
- Procedure for the rapid, large-scale purification of Escherichia coli DNA-dependent RNA polymerase involving polymin P precipitation and DNA-cellulose chromatographyBiochemistry, 1975
- The role of replication proteins in the regulation of bacteriophage T4 transcription: I. Gene 45 and hydroxymethyl-C-containing DNAJournal of Molecular Biology, 1975
- Bacteriophage T4 transcriptional control gene 55 codes for a protein bound to Escherichia coli RNA polymeraseJournal of Molecular Biology, 1974
- The interaction of bacterial and phage proteins with immobilized Escherichia coli RNA polymeraseJournal of Molecular Biology, 1974
- Polypeptide Bound to the Host RNA Polymerase is Specified by T4 Control Gene 33Nature New Biology, 1973
- Deoxyribonucleic acid replication in vitroJournal of Molecular Biology, 1972
- A New Method of Large Scale Preparation of Highly Purified DNA- Dependent RNA-Polymerase fromE. coliHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1970
- A New Method for the Large Scale Purification of Escherichia coli Deoxyribonucleic Acid-dependent Ribonucleic Acid PolymeraseJournal of Biological Chemistry, 1969
- In vitro synthesis of T4 late messenger RNA.Proceedings of the National Academy of Sciences, 1968
- EARLY ENZYME SYNTHESIS AND ITS CONTROL IN E. COLI INFECTED WITH SOME AMBER MUTANTS OF BACTERIOPHAGE T4Proceedings of the National Academy of Sciences, 1962