Fate of donor insertion sequence IS1 during transposition.
- 1 May 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (5) , 2514-2518
- https://doi.org/10.1073/pnas.77.5.2514
Abstract
The number of insertion sequence IS1 segments in Escherichia coli K-12 and 20 mutants in which an IS1 was inserted at a new site was measured by Southern blot hybridization analysis. The parent strain appeared to contain seven IS1 segments. Each of the mutants contained these 7 IS1 and 1 additional IS1 corresponding to the new IS1 insertion. A copy of a donor IS1 is inserted at the new site. A model for transposition is presented that postulates that a reactive intermediate is formed by a tandem duplication of a transposable sequence.This publication has 34 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Polymorphisms in the chromosomal locations of elements of the 412, copia and 297 dispersed repeated gene families in drosophilaCell, 1979
- Physical structures of Tn10-promoted deletions and inversions: Role of 1400 bp inverted repetitionsCell, 1979
- Nearly precise excision: a new type of DNA alteration associated with the translocatable element Tn10Cell, 1979
- Mapping unintegrated avian sarcoma virus DNA: Termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNACell, 1978
- Proviruses of avian sarcoma virus are terminally redundant, co-extensive with unintegrated linear DNA and integrated at many sitesCell, 1978
- DNA Sequence of the mini-insertion IS2–6 and its relation to the sequence of IS2Nature, 1978
- DNA sequence at the integration sites of the insertion element IS1Cell, 1978
- DNA REARRANGEMENTS IN PROCARYOTESAnnual Review of Genetics, 1977
- Organization of ribosomal protein genes in Escherichia coli: I. Physical structure of DNA from transducing λ phages carrying genes from the aroE-str regionJournal of Molecular Biology, 1976