Studies on the 'Osmophilic' Yeast Saccharomyces rouxii and an Obligate Osmophilic Mutant

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Abstract
SUMMARY An obligate osmophilic mutant (strain BI /4) of Saccharomyces rouxii has been isolated that fails to grow at osmotic pressures corresponding to 20 % (w/v) sucrose or less. In 30 % sucrose the yeast is filamentous and grows slowly. In 40 % sucrose it is mainly filamentous and has over twice the normal diameter. In 60 % sucrose it grows in the yeast form with a growth rate twice that of the culture in 40 % sucrose. This mutant is lysed by a sudden drop in the osmotic pressure of the environment. Cell envelopes of the parent strain contained glucose and mannose in the ratio 1-2: I and contained 3-8 % (w/v) hexosamine, whereas the envelopes of the mutant contained 0.8 % hexosamine. Cell envelopes of the mutant grown in 40 % sucrose contained glucose and mannose in the ratio 1.9: I, whereas for envelopes of the yeast grown in 60 % sucrose the ratio was 1.2: I. Neutral lipids from whole cells and those from the envelopes of the mutant strain generally contained more unsaturated fatty acids than the corresponding fractions from the parent strain.

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