Cytotoxic antibodies to chicken adipocytes and their precursors: Lack of tissue specificity

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Abstract
1. Antisera raised in sheep against chicken adipocyte plasma membranes recognised adipocyte, liver and red blood cell membranes in enzyme immunoassays (EIA). 2. These antisera were cytotoxic when incubated with both adipocytes and their precursors grown in culture, and with red blood cells. 3. Adsorption of antisera with liver membranes completely abolished the response to liver and red blood cell membranes in the EIA. The response in adipocytes was reduced to only about 6% of the titre present in the unadsorbed sample. 4. Adsorption of antisera with liver membranes abolished the cytotoxic response to red blood cells and to adipocyte precursors, but some response to adipocytes still remained. 5. These results are in contrast to the rat for which adipocyte specific antisera were obtained which represented about 75% of the unadsorbed titre.