Selection oflonmutants inEscherichia coliby treatment with phenothiazines

1 August 1977

journal article
research article
Published by Hindawi Limited in Genetics Research

Vol. 30 (1) , 13-20
https://doi.org/10.1017/s0016672300017420

Abstract

SUMMARY: Growth ofE. coliK12 in the presence of chlorpromazine or promethazine resulted in the appearance of a special kind of mutational change with a frequency of 5–10%. These cells were UV-sensitive and very mucoid in their colony formation. The strains were characterized as colanic acid producing on the basis of phage m-59 sensitivity and we tentatively conclude that these mutants are Lon⁻.In another series of experiments drug treatment ofE. colifailed to yield significant numbers of auxotrophs and the back mutation rate of a number ofS. typhimuriumHis⁻strains was not significantly increased.

Keywords

ESCHERICHIA COLI

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