An enzyme-linked oligonucleotide assay
- 1 August 1996
- journal article
- Published by Springer Nature in Nature Biotechnology
- Vol. 14 (8) , 1021-1025
- https://doi.org/10.1038/nbt0896-1021
Abstract
The recent development of in vitro methods to select high-affinity ligands by combinatorial chemistry methodologies promises unique and theoretically unlimited supplies of novel therapeutic and diagnostic reagents. One such combinatorial chemistry process, systematic evolution of ligands by exponential enrichment (SELEX), allows rapid identification, from large random sequence pools, of the few oligonucleotide sequences that bind to a desired target molecule with high affinity and specificity. We describe an enzyme-linked sandwich assay that uses a SELEX-derived oligonucleotide. This assay demonstrates that these oligonucleotides can be effective and useful analytical reagents.Keywords
This publication has 43 references indexed in Scilit:
- Selexion: Systematic evolution of ligands by exponential enrichment with integrated optimization by non-linear analysisPublished by Elsevier ,2005
- In Vitro Selection of RNA Ligands to Substance PBiochemistry, 1995
- Diversity of Oligonucleotide FunctionsAnnual Review of Biochemistry, 1995
- RNAs with Dual Specificity and Dual RNAs with Similar SpecificityScience, 1994
- RNA Selection: Aptamers achieve the desired recognitionCurrent Biology, 1994
- Significance of Vascular Endothelial Growth Factor/Vascular Permeability Factor for Solid Tumor Growth, and Its Inhibition by the AntibodyBiochemical and Biophysical Research Communications, 1993
- Inhibition of vascular endothelial growth factor-induced angiogenesis suppresses tumour growth in vivoNature, 1993
- In vitro selection of RNA molecules that bind specific ligandsNature, 1990
- Systematic Evolution of Ligands by Exponential Enrichment: RNA Ligands to Bacteriophage T4 DNA PolymeraseScience, 1990
- Enzyme-linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin GImmunochemistry, 1971