Dose—time relationship of the development of preneoplastic liver lesions induced in rats with low doses of N-nitrosodiethanolamine

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Abstract
The carcinogenic efficiency of very low doses of N-nitroso-diethanolamine (NDELA), an N-nitroso compound of environmental significance, was assessed by administering it to male Sprague—Dawley rats at five different dose levels: 0.2, 0.63, 1.5, 6 and 25 mg/kg b.w./day in the drinking water. Quantitation of the numbers and size of liver foci positive for glucose-6-phosphate dehydrogenase (G6PDH) by morpho-metric methods revealed a good correlation between the dose and duration of carcinogen treatment and the extent of G6PDH-positive foci development. Thus the area density increased proportional to time and dose. The dose—time relation for the induction of 1% G6PDH-positive liver tissues assessed as a double logarithmic plot gives a straight line with the same characteristics as that which results when the induction of liver tumors is evaluated.