Regulation of uncoupling protein activity in phosphorylating potato tuber mitochondria
- 21 July 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (20) , 4437-4442
- https://doi.org/10.1016/j.febslet.2005.07.008
Abstract
In isolated potato tuber mitochondria, palmitic acid (PA) can induce a H+ leak inhibited by GTP in the phosphorylating (state 3) respiration but not in the resting (state 4) respiration. The PA-induced H+ leak is constant when state 3 respiration is decreased by an inhibition of the succinate uptake with n-butyl malonate (nBM). We show that the efficiency of inhibition by GTP is decreased when state 3 respiration is progressively inhibited by antimycin A (AA) and is restored following subsequent addition of nBM. We propose that in phosphorylating potato tuber mitochondria, the redox state of ubiquinone, which can antagonistically be varied with AA and nBM, modulates inhibition of the PA-activated UCP-sustained H+ leak by GTP.Keywords
This publication has 23 references indexed in Scilit:
- Activation and Function of Mitochondrial Uncoupling Protein in PlantsJournal of Biological Chemistry, 2004
- Mitochondrial superoxide: production, biological effects, and activation of uncoupling proteinsPublished by Elsevier ,2004
- Possible role of free oxidation processes in the regulation of reactive oxygen species production in plant mitochondriaBiochemical Society Transactions, 2003
- Superoxide Stimulates a Proton Leak in Potato Mitochondria That Is Related to the Activity of Uncoupling ProteinJournal of Biological Chemistry, 2003
- Superoxide activates mitochondrial uncoupling proteinsNature, 2002
- Involvement of the Dicarboxylate Carrier in the Protonophoric Action of Long-Chain Fatty Acids in MitochondriaBiochemical and Biophysical Research Communications, 1997
- Role of uncoupled and non-coupled oxidations in maintenance of safely low levels of oxygen and its one-electron reductantsQuarterly Reviews of Biophysics, 1996
- The Relationship Between Electron Flux and the Redox Poise of the Quinone Pool in Plant MitochondriaEuropean Journal of Biochemistry, 1994
- The ATP/ADP‐antiporter is involved in the uncoupling effect of fatty acids on mitochondriaEuropean Journal of Biochemistry, 1989
- Membrane potential of mitochondria measured with an electrode sensitive to tetraphenyl phosphonium and relationship between proton electrochemical potential and phosphorylation potential in steady stateThe Journal of Membrane Biology, 1979