Kinetics and mechanism of catalysis by proteolytic enzymes: The kinetics of hydrolysis of derivatives of l-lysine and S-(β-aminoethyl)-l-cysteine(thialysine)by bovine trypsin
- 1 March 1967
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 102 (3) , 728-734
- https://doi.org/10.1042/bj1020728
Abstract
Several esters of the [alpha] -N-toluene-p-sulphonyl and N-benzoyl derivatives of L-lysine and S-([beta]-aminoethyl)-L-cysteine have been synthesized. The kinetics of hydrolysis of the esters by bovine trypsin have been compared. Values of k0 are similar for corresponding derivatives of the isosteric amino acids and deacylation of an acyl-enzyme appears to be rate-determining in each case. There are, however, some quantitative kinetic differences between the various series of substrates.This publication has 7 references indexed in Scilit:
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