Comparison of human papillomavirus Type 1 serotyping by monoclonal antibodies with genotyping by in situ hybridization of plantar warts

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Abstract
Thirty plantar warts were analyzed for the presence of HPV‐1 type‐specific and PV genus‐specific capsid antigens by immunofluorescence (IF) using monoclonal and polyclonal antibodies and type‐specific HPV‐1 DNA employing in situ hybridization methods. Fifteen of 30 plantar warts were positive by IF for PV genus‐specific structural viral antigens. Thirteen of the 15 productively infected plantar warts expressed intranuclear HPV‐1 type‐specific capsid antigens and viral DNA, which were detected in the same distribution in each individual wart. The 2 productively infected plantar warts that did not react with HPV‐1 type‐specific MoAbs did not react with HPV‐1 type‐specific DNA by in situ hybridization. Thus, serotyping of HPV‐1 capsid antigens by monoclonal antibodies is concordant with genotyping of HPV‐1 viral DNA by in situ hybridization in productively infected plantar warts.