Immunoperoxidase techniques: the deleterious effect of sodium azide on the activity of peroxidase conjugates.

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Abstract
The effect of including sodium azide as a bacteriostatic agent in solutions used to dilute [rabbit] antibodies conjugated with the enzyme horseradish peroxidase was examined. An enzyme-linked immunosorbent assay and an immunohistochemical method were used and both techniques demonstrated an inhibitory effect of sodium azide on the activity of the peroxidase conjugates. The use of sodium azide in solutions used to dilute peroxidase conjugates should be avoided.