LEGIONELLA EXTRACELLULAR PROTEASE ACTIVITY ON CHROMOGENIC PEPTIDES: A SIMPLIFIED PROCEDURE FOR BIOCHEMICAL ENZYME IDENTIFICATION

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Abstract
Legionella strains produce extracellular proteases. One method to demonstrate these is through the activity of broth culture filtrates on para‐nitroanilide (pNA)‐derivatized peptides. Previously, this method has comprised a 100‐times concentration of the filtrates, in order to bring the protease activity, as measured by the liberation of free pNA from hydrolysed peptides, up to easily recordable values. By introducing a diazotation step, the sensitivity of the test has been increased sufficiently to omit the time‐consuming concentration procedure. Accordingly, the analysis of extracellular Legionella proteases by pNA‐derivatized peptides has become rapid and straight‐forward.

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