Dichlorophenylurea-insensitive Reduction of Silicomolybdic Acid by Chloroplast Photosystem II
Open Access
- 1 March 1975
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 55 (3) , 460-462
- https://doi.org/10.1104/pp.55.3.460
Abstract
The photoreduction of silicomolybdate and other heteropoly ions by chloroplasts is insensitive to 3-(3, 4-dichlorophenyl)-1, 1-dimethylurea (DCMU). Both water and diphenylcarbazide can be used as electron source for the reduction. Three different assays for silicomolybdate reduction are described including oxygen evolution, formation of a reduced heteropoly blue silicomolybdate, or an indirect assay for reduced silicomolybdate by redox indicators, such as ferricyanide or cytochrome c. The effects of detergents and tris washing are consistent with silicomolybdate reduction through photosystem II before the DCMU site. The effects of orthophenanthroline and bathophenanthroline indicate chelator-sensitive sites in photosystem II before the site of DCMU action.Keywords
This publication has 6 references indexed in Scilit:
- Photophosphorylation not coupled to DCMU-insensitive photosystem II oxygen evolutionBiochemical and Biophysical Research Communications, 1974
- Studies on electron transport associated with photosystem I. III. The reduction sites of various hill oxidants in the photosynthetic electron transport systemBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1973
- Selective Inhibition of Photosynthetic Reactions by PolycationsJournal of Biological Chemistry, 1972
- Inhibition of the Hill Reaction by Tris and Restoration by Electron Donation to Photosystem IIPlant Physiology, 1969
- COFACTORS AND RATES OF PHOTOSYNTHETIC PHOSPHORYLATION BY SPINACH CHLOROPLASTSJournal of Biological Chemistry, 1958
- Studies on metalloflavoproteinsBiochimica et Biophysica Acta, 1956