DNA sequence components of the Pinusstrobus nuclear genome

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Abstract
The nuclear DNA of Pinusstrobus L. was characterized by whole-genome hydroxyapatite reassociation kinetics. The genome, which is very large, is not well described by partition into three, four, or five major kinetic components. Instead, it consists of a large array of sequence components with kinetics suggesting a more or less continuous range of frequencies from very large to a single copy. The single-copy fraction, which is estimated to be about 24% of the total, appears to fit the general hypothesis for large genomes, i.e., most of it probably consists of ancient diverged repeated sequences. Under standard conditions of DNA reassociation kinetics, these sequences are not completely diverged but react with kinetics indicating that there is an average of about three repeats for the fraction. It is estimated that only about 0.1% of white pine nuclear DNA is expressed as mRNAs.
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