Role of cloned carotenoid genes expressed in Escherichia coli in protecting against inactivation by near-UV light and specific phototoxic molecules
Open Access
- 1 October 1988
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 170 (10) , 4675-4680
- https://doi.org/10.1128/jb.170.10.4675-4680.1988
Abstract
Genes controlling carotenoid synthesis were cloned from Erwinia herbicola and expressed in an Escherichia coli strain. Carotenoids protect against high fluences of near-UV (NUV; 320 to 400 nm) but not against far-UV (200-300 nm). Protection of E. coli cells was not observed following treatment with either psoralen or 8-methoxypsoralen plus NUV. However, significant protection of cells producing carotenoids was observed with three photosensitizing molecules activated by NUV (alpha-terthienyl, harmine, and phenylheptatriyne) which are thought to have the membrane as an important lethal target. Protection of carotenoid-producing cells against inactivation was not observed with acridine orange plus visible light but was seen with toluidine blue O plus visible light.This publication has 48 references indexed in Scilit:
- A COMPARISON OF PHOTO-KILLING AMONG CAROTENE AND CYTOCHROME c ACCUMULATING STRAINS OF THE SMUT FUNGUS Ustilago violacea AT SPECIFIC WAVELENGTHS FROM 400 TO 650 nmPhotochemistry and Photobiology, 1987
- CAROTENE AND CYTOCHROME c DEPENDENT MITOTIC RECOMBINATION AND KILLING IN RESPONSE TO VISIBLE LIGHT AND UV‐C IN THE SMUT FUNGUS Ustilago ViolaceaPhotochemistry and Photobiology, 1987
- Inactivation and mutagenesis by phototoxins usingEscherichia coli strains differing in sensitivity to near- and far-ultraviolet lightJournal of Chemical Ecology, 1986
- Insect defenses against phototoxic plant chemicalsJournal of Chemical Ecology, 1986
- Light-mediated allelochemical effects of naturally occurring polyacetylenes and thiophenes from asteraceae on herbivorous insectsJournal of Chemical Ecology, 1986
- EXCITED STATE PROPERTIES OF ∝aL‐TERTHIENYL and RELATED MOLECULESPhotochemistry and Photobiology, 1985
- MEMBRANE DAMAGE CAN BE A SIGNIFICANT FACTOR IN THE INACTIVATION OF ESCHERICHIA COLI BY NEAR‐ULTRAVIOLET RADIATIONPhotochemistry and Photobiology, 1981
- PHOTODYNAMIC AND SUNLIGHT INACTIVATION OF ESCHERICHIA COLI STRAINS DIFFERING IN NEAR‐UV SENSITIVITY AND RECOMBINATION PROFICIENCYPhotochemistry and Photobiology, 1980
- GENETIC CONTROL OF NEAR‐UV (300–400nm) SENSITIVITY INDEPENDENT OF THE recA GENE IN STRAINS OF ESCHERICHIA COLI K12Photochemistry and Photobiology, 1979
- Concerning the use of amines as probes for participation of singlet oxygen in dye-sensitised oxygenation reactionsJournal of the Chemical Society, Perkin Transactions 2, 1977