Use of 2-Methyl-6-phenyl-3, 7-dihydroimidazo[1, 2-a]-pyrazin-3-one for Assay of Superoxide Anions in Phagocytizing Granulocytes
- 1 January 1986
- journal article
- Published by The Society for Free Radical Research Japan in Journal of Clinical Biochemistry and Nutrition
- Vol. 1 (1) , 5-10
- https://doi.org/10.3164/jcbn.1.5
Abstract
As a chemiluminescence probe, 2-methyl-6-phenyl-3, 7dihy-droimidazo[1, 2-a]pyrazin-3-one was used to detect superoxide anion generation in granulocytes during phagocytosis. The reaction of this probe is highly specific for and sensitive to superoxide anions and can be adopted to determine them in terms of xanthine oxidase units.Generation of superoxide anions in response to opsonized zymosan was checked for granulocytes of peripheral blood samples from 9 adults and those of cord blood samples from 11 neonates. The ability to generate superoxide anions during phagocytosis was found to be greater in granulocytes of cord blood from neonates than in those of peripheral blood from adults.Keywords
This publication has 0 references indexed in Scilit: