The persistence and precise location of Ancylostoma caninum larvae in tissues of vertebrate paratenic hosts and the nature of host responses were studied in mouse, cat, and monkey. Mice were infected percutaneously and examined at various intervals up to 260 days after exposure. Long-persisting larvae were found only in the muscles. Histologic sections revealed that within 4 hr after exposure some larvae had migrated through the skin and had entered individual fibers of the underlying muscles. After the 1st day nearly all larvae found in muscles were within fibers. Granuloma formation and encapsulation were not observed, suggesting that inside the fibers the larvae produced no direct inflammatory reaction. Only diffuse infiltration of inflammatory cells was observed in muscles and this appeared to be in response to destruction of muscle fibers. Larvae were similarly located in muscles of a cat and a rhesus monkey examined 16 and 17 days, respectively, after cutaneous exposure. The histologic changes observed in muscle fibers invaded by A. caninum larvae are similar to those observed in early Trichinella spiralis infections. The larvae of A. canninum lying coiled within the muscle fibers also bear superficial resemblance to the larvae of T. spiralis. A. caninum larvae were also recovered by tissue digestion from muscles of naturally infected dogs, which suggests that larvae reside in this location in the bitch prior to transfer to the neonate via the milk.