Skin Penetration Method Suggested for Environmental Protection Agency Requirements

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Abstract
The Environmental Protection Agency has circulated a protocol for examining the dermal absorption of pesticides in rats. This protocol will be considered as a guideline for determining the dermal absorption of pesticides. Approximately 40 pesticides have been evaluated with this protocol. Male rats are dosed dermally with labeled pesticide. Doses, in mg/cm2, are applied to the shaven skin of the back as the use product, diluted with water if necessary. The application site is protected with a nonocclusive device. Four rats per dose are exposed for 0.5, 1, 2, 4, 10, or 24 hrs. Samples collected are soap and water wash, skin at the application site, blood, total urine and feces, carcass, and selected tissues. Mass balance calculations include determination of pesticide that can be removed with soap and water, pesticide bound on or in the skin, total pesticide absorbed with time, blood concentrations with time, pesticide accumulation in target tissues, and pesticide excreted.
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