Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

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Abstract
Phospholipid analyses were made on whole cells and isolated plasma membranes (PM) of 6 different lines of Yoshida ascites hepatoma and normal liver. The hepatoma PM had lower percentage levels of choline phosphoglycerides and phosphatidylinositol, and higher percentages of sphingomyelin, phosphatidylethanolamine and phosphatidylserine in total phospholipids than liver PM. Similar tendencies were observed in the whole cell phospholipids, although such trends were not observed in the mitochondrial or microsomal fractions. The lysophospholipid percentages in hepatoma PM were lower than those in liver PM. Hepatomas had higher percentage levels of plasmalogen in total phospholipids than normal liver, in both whole cells and PM. PM from island-forming hepatomas contained more plasmalogen than PM from their free-cell sublines. Localization of plasmalogen was observed in PM and certain intracellular organelles of island-forming hepatomas by histochemical plasmal reaction.