Nucleotide Sequence of the 5' Flanking Region Responsible for the Enhancement of the Expression of Yeast Enolase 1 Gene1

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Abstract
Several plasmids carrying different length of the 5' flanking region of yeast (Saccharomyces cerevisiae) enolase 1 gene (ENO1) which is fused in frame to the Escherichia coli lacZ gene were constructed by recombination in vitro. Promoter activity of ENO1 was assayed by measuring β-galactosidase activity of the fused gene product. Comparison of the promoter activity of these plasmids suggests that the sequences required for a strong promoter activity lie within the DNA segment −724 to −353 base pairs (bp) upstream from the start of ENO1 coding sequence. The nucleotide sequence of this region was determined.