QUANTITATION OF SERUM FERRITIN BY ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA)

  • 1 January 1980
    • journal article
    • research article
    • Vol. 57  (8) , 282-287
Abstract
An enzyme-linked immunosorbent assay (ELISA) was developed for determining ferritin in serum. In this assay the solid phase consists of rabbit antiferritin Ig[immunoglobulin]G adsorbed onto PVC microtitre plates. Ferritin from standards or samples binds to the solid phase and is detected by a conjugate consisting of rabbit antiferritin-IgG and horseradish peroxidase. The entire assay can be performed in 5 h, with good precision. The assay has a useful range of 1 to 64 .mu.g/l and serum samples must be diluted 10-fold. The coefficient of variation [r] for 6 samples (1,5- 40,1 .mu.g/l) assayed 12 times on the same plate varied from 4 to 9% (intra-assay). When these 6 samples were assayed on different plates and on different days by 2 technicians the r value varied from 4 to 11% (inter-assay). The lowest detectable level of ferritin was 1 .mu.g/l. Forty-six serum samples from blood donors were assayed by the method described here (y) and compared with results obtained by HIA [radioimmunoassay] (x). The resulting regression equation was y = 1073x - 4.33; r = 0.984.

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