Improved Manual Spectrophotometric Procedure for Determination of Serum Triglycerides
Open Access
- 1 September 1973
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 19 (9) , 1077-1078
- https://doi.org/10.1093/clinchem/19.9.1077
Abstract
We have modified the procedure of Levy [Ann. Clin. Lab. Sci. 6, 474 (1972)] for determination of serum triglycerides. The changes consist of using heptane in place of nonane; using concentrated aqueous potassium hydroxide instead of sodium methylate as the saponifying reagent; and a somewhat higher saponification, oxidation, and condensation temperature. Consequently, the yellow dehydrolutidine derivative is determined in a homogeneous solution having an almost linear absorbance up to 3 g/liter.Keywords
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