Analysis of the growth ofChlorellacultures using electronically measured cell-volume distribution data

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Abstract
Cell-number density and cell-volume distribution data were obtained from cultures of C. fusca var. vacuolata Shihira et Krauss growing under both continuous and periodic illumination. Mean, median and modal cell-volumes were calculated from the cell-volume distributions and a high correlation shown between mean and median values, while mean and modal values did not correlate well. Where the computation necessary for deriving mean cell-volumes was not practicable, the median cell-volume was the most useful statistic. Synchrony indices of discontinuously illuminated cultures gave similar values when based on change in cell-number and change in mean cell-volume. Cultures under continuous illumination showed synchronous divisions at the beginning of growth. These divisions gave high values of a synchrony index based on change in cell-number, but low values of an index based on change in mean cell-volume. Mean cell-volume data were more sensitive to the occurrence of unbalanced growth than analysis of cell-number data.
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