Synthesis of Transfer RNA during the Synchronous Nuclear Division Cycle in Physarum polycephalum

Abstract
The synthesis of tRNA during the synchronous nuclear division cycle in plasmodia of P. polycephalum was investigated using an isotope dilution procedure and compared to the pattern of synthesis of rRNA. The synthesis of both tRNA and rRNA stops during mitosis. As the genes for the 2 types of RNA are not linked, this finding suggests that cessation of synthesis during nuclear division is a common characteristic of all types of transcription, supporting earlier work using pulse labeling and autoradiography to investigate the same problem.