ACCELERATED CELLULAR-RECOVERY AFTER AN ISCHEMIC RENAL INJURY

  • 1 January 1983
    • journal article
    • research article
    • Vol. 112  (3) , 338-346
Abstract
To determine the mode of action of the beneficial effect of ATP MgCl2, recovery of microinjected inulin, proximal tubular pressure (PTP), and cellular damage as quantitated by histomorphometric analysis of necrosis and swelling were evaluated at 2, 6 and 24 h after 45 min of renal ischemia in rats treated with either normal saline or ATP-MgCl2. At 2 h both groups of rats demonstrated increased permeability to inulin, elevated PTP, and severe ischemic damage and necrosis. By 6 h ATP-MgCl2 rats had less tubular back leak of inulin, PTP was modestly reduced, and ultrastructural studies demonstrated improved cellular morphologic features with evidence of early regenerative changes. The saline rats had progressive ischemic cellular damage. At 24 h ATP-MgCl2 rats had reestablished tubular integrity, PTP had fallen, and ischemic alterations were improved, with only focal evidence of necrosis. Saline treated rats still had a back leak of inulin, elevated PTP, and progressive ischemic injury. Cellular damage continues to occur for 6 h after renal ischemia. ATP-MgCl2 enhances recovery of tubular integrity and cellular morphologic features. The salutary effect of ATP-MgCl2 appears related to the preservation of sublethally injured cells and acceleration of the process of restoration and repair of damaged cells.