TRANSPLANTATION SYSTEM FOR DETERMINING THE CLONOGENIC SURVIVAL OF PARENCHYMAL HEPATOCYTES EXPOSED TO IONIZING-RADIATION

  • 1 January 1981
    • journal article
    • research article
    • Vol. 41  (9) , 3512-3518
Abstract
An in vivo transplantation technique has been developed which can be utilized to determine the reproductive survival of [rat] parenchymal hepatocytes exposed in vitro or in vivo to physical and chemical genotoxic agents. This assay system was used to determine the survival of liver cells exposed to sparsely ionizing radiation. The D0 [mean lethal dose] value of the survival curve was 249 rad and the extrapolation number was 1.2. Hepatocytes irradiated while in the G0 phase are apparently unable to accumulate sublethal damage to an appreciable extent if they are stimulated to undergo replication within 24 h after the infliction of the damage. [This system is being used for carcinogenesis studies.].