Metabolism of phosphoenolpyruvate in the C4 cycle during photosynthesis in the phosphoenolpyruvate-carboxykinase C4 grass Spartina anglica Hubb.
- 1 December 1983
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 159 (6) , 570-578
- https://doi.org/10.1007/bf00409147
Abstract
The aim of this work was to investigate the fate of phosphoenolpyruvate (PEP) produced by decarboxylation of oxaloacetate during photosynthesis in the bundle sheaths of leaves of the PEP-carboxykinase C4 grass Spartina anglica Hubb. Mesophyll protoplasts and bundle sheath cells were separated enzymically and used to investigate activities and distributions of putative enzymes of the C4 cycle and the photosynthetic carbon metabolism of bundle sheath cells. The results indicate that neither conversion of PEP to pyruvate nor its conversion to 3-phosphoglycerate can account for all of the carbon flux through the C4 cycle during photosynthesis. It is likely, therefore, either that PEP moves directly from bundle sheath to mesophyll or that more than one pathway of regeneration of PEP is involved in the C4 cycle in this plant.Keywords
This publication has 20 references indexed in Scilit:
- Intracellular location of phosphoenolpyruvate carboxykinase and other C4 photosynthetic enzymes in mesophyll and bundle sheath protoplasts of Panicum maximumPlant Science Letters, 1983
- Influence of Oxygen and Temperature on the Dark Inactivation of Pyruvate, Orthophosphate Dikinase and NADP-Malate Dehydrogenase in MaizePlant Physiology, 1983
- Dark-light regulation of pyruvate, Pi dikinase in C4 plants: Evidence that the same protein catalyses activation and inactivationBiochemical and Biophysical Research Communications, 1983
- Isozymes of the Glycolytic Enzymes in Endosperm from Developing Castor Oil SeedsPlant Physiology, 1982
- Characterization and Kinetics of Isoenzymes of Pyruvate Kinase from Developing Castor Bean EndospermPlant Physiology, 1980
- Isoenzymes of Pyruvate Kinase in Etioplasts and ChloroplastsPlant Physiology, 1979
- Photosynthetic activities of isolated bundle sheath cells in relation to differing mechanisms of C4 pathway photosynthesisArchives of Biochemistry and Biophysics, 1976
- Regulation of Oxaloacetate, Aspartate, and Malate Formation in Mesophyll Protoplast Extracts of Three Types of C4 PlantsPlant Physiology, 1975
- Phosphoenolpyruvate carboxykinase in leaves of certain plants which fix CO2 by the C4-dicarboxylic acid cycle of photosynthesisBiochemical and Biophysical Research Communications, 1971
- COPPER ENZYMES IN ISOLATED CHLOROPLASTS. POLYPHENOLOXIDASE IN BETA VULGARISPlant Physiology, 1949