Disopyramide determination by gas chromatography, liquid chromatography, and gas chromatography--mass spectrometry.
- 1 November 1979
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 25 (11) , 1900-1904
- https://doi.org/10.1093/clinchem/25.11.1900
Abstract
Disopyramide is determined in serum by gas chromatography with a nitrogen-selective detector, by liquid chromatography, and by gas chromatography--mass spectrometry. Comparable results are obtained with the three techniques, with a within-run and between-run precision of 5 to 10% (coefficient of variation). Least-squares analysis of data on patients' sera, analyzed first by gas chromatography (y) and then liquid chromatography (x), gave a slope of 1.12; y-intercept, -0.31; standard error of estimate, 0.46; and correlation coefficient, 0.94. Comparison of patients' sera by gas chromatography (y) and then by gas chromatography--mass spectrometry (x) gave a slope of 0.94; y-intercept, 0.42; standard error of estimate, 0.38; and correlation coefficient, 0.97. Interferences observed when using one technique--for example, gas chromatography--can be eliminated by analyzing the sample extract with one of the other techniques.This publication has 4 references indexed in Scilit:
- Fluorescence photometric determination of disopyramide and mono-N-dealkylated disopyramide in plasma after separation by thin-layer chromatographyAnalytical Chemistry, 1979
- Gas—liquid chromatographic method for the routine estimation of disopyramide in plasma or serumJournal of Chromatography A, 1978
- Simple gas-liquid chromatographic method for the measurement of disopyramide in blood-plasma or serum and in urineJournal of Chromatography A, 1978
- High-pressure liquid chromatographic analysis of drugs in biological fluidsJournal of Chromatography A, 1977