Active Sodium Transport Across the Epithelium of the Human Kidney Pelvis. Part 1

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Abstract
To clarify whether the epithelium of the human urinary tract possesses an active transepithelial Na transport, the unidirectional Na fluxes and the electrical parameters of the epithelium were determined on isolates pieces of renal pelvis. The epithelium was incubated in isotonic Ringer''s solution at 37.3C in Ussing-type chambers. The transepithelial potential difference was 15.8 .+-. 3.0 mV and the transepithelial electrical resistance 760 .+-. 48 .OMEGA. .cntdot. cm-2. The transepithelial, unidirectional Na fluxes were measured under short-circuit conditions. From both the unidirectional Na influx from the luminal to the serosal side (7.01 .+-. 2.17) and from the efflux in the opposite direction (0.45 .+-. 0.07) a transepithelial Na net flux of 6.56 .+-. 2.20 nmol/min. cm-2 was calculated. Since there are no passive forces across the epithelium under short circuited conditions to explain the net flux, an active Na transport has to be postulated. In all experiments, the simultaneously measured short circuit current (6.43 .+-. 2.18 nmol/min cm-2) was not significantly different from the net Na flux, and can be taken as a measure of the net flux.

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