Effects of Nutrition on Aspartic Acid Deaminase System of Bacterium cadaveris

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Abstract
The effect of the concn. of the constituents of the growth medium on the aspartic acid deaminase system of B. cadaveris was studied. The basal medium (1% each of yeast extract and pepticase, and 0.5% K2HPO4) was varied by holding the concn. of 2 ingredients constant and using 0, 1, 3 and 5% of the other constituent in parallel expts. The deaminase activity of the harvested cells before washing and after 1, 2, and 3 successive washes in dist. H2O was detd. The aspartic acid deaminase activity of the cell suspensions before washing was not altered by varying the concn. of any one of the constituents of the growth medium from 0-5%. However, marked differences were noted after 2 and 3 washings in dist. H2O. The activity after 3 washings in distilled H2O increased in direct proportion to the concn. of either yeast extract or pepticase in the growth medium, while for phosphate the greatest activity appeared at a concn. of 1% with a definite decrease in activity below and above this concn.

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