Solution 1H NMR Characterization of Equilibrium Heme Orientational Disorder with Functional Consequences in Mouse Neuroglobin
- 14 June 2003
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 125 (27) , 8080-8081
- https://doi.org/10.1021/ja034584r
Abstract
The solution 1H NMR spectrum of oxidized (met) mouse neuroglobin, metNgb, demonstrates that it is low-spin and hexacoordinate with strong spectral similarities to ferricytochrome b5. The axial ligands are identified as His(F8) and His(E7), with the latter exhibiting an unstrained Fe-His bond. The presence of two sets of resonances is shown to arise from equilibrium heme orientational isomers ( approximately 2:1). The ligation of cyanide is shown to be extraordinarily slow with a factor approximately 2 difference in rate for the two heme orientations. Not only is Ngb the first mamalian globin with equilibrium heme disorder, but the disorder also has additional functional consequences.Keywords
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