Quantitative Analysis of Adsorbed Serum Albumin on Segmented Polyurethane Using FT-IR/ATR Spectroscopy

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Abstract
FT-IR attenuated total reflection (ATR) spectroscopy was applied to quantitatively determine the extent of bovine serum albumin adsorbed onto a biomedical-grade poly(ether)urethane film deposited on a ZnSe internal reflection element (IRE). The method of adsorption density determination was based on the optical principles for a stratified medium consisting of three layers. The spectral peak area due to bulk solution was less than 1% of total peak area. The measured adsorption density of albumin in a flowing system was 3.9 μg/cm2 at a solution concentration of 4.5 g/100 mL.