Screening Plate Method for Detection of Bacterial β-Glucuronidase
Open Access
- 1 January 1973
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 26 (6) , 863-866
- https://doi.org/10.1128/aem.26.6.863-866.1973
Abstract
For screening tests of bacterial β-glucuronidase (EC 3.2.1.31) activity an agar plate (MUG plate) containing the enzyme substrate, 4-methylumbelliferyl-β-D-glucuronide, was developed. In the presence of β-glucuronidase, the strongly fluorescent 4-methylumbelliferone, which can be detected by ultraviolet light, was liberated. For tests of fastidious bacteria, the MUG-plate was enriched with supplements containing heat-labile growth factors without influencing the reaction. The plate was found to be suitable for screening tests. The sensitivity of the MUG-plate was determined by means of fluorimetric method. With the present method, β-glucuronidase activities were demonstrated in some well-known β-glucuronidae-producing microorganisms as well as in Bacteroides and Corynebacterium spp. ImagesKeywords
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