Nitric Oxide Production in the CA1 Field of the Gerbil Hippocampus After Transient Forebrain Ischemia

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Abstract
Background and Purpose—The present study was designed to examine the time course of nitric oxide (NO) production and the source of NO in the CA1 field of the gerbil hippocampus after transient forebrain ischemia. Methods—The production of NO in the CA1 field of the hippocampus after transient ischemia was monitored consecutively by measuring total NO metabolites (NOx−, NO2− plus NO3−) with the use of brain microdialysis. 7-Nitroindazole (7-NI) and NG-nitro-l-arginine methyl ester were used to dissect the relative contributions of neuronal NO synthase and endothelial NO synthase to the NO production. The histological outcomes of 7-NI in 5- and 10-minute global ischemia were also evaluated. Results—The production of NO in the CA1 field of the hippocampus after ischemia was dependent on the severity of ischemia. Ischemia for 2 or 5 minutes did not induce a significant increase in NOx− levels in the CA1 field of the hippocampus after reperfusion, whereas the 10- and 15-minute ischemias produced significant an...