Multiple sites of HOX‐7 expression during mouse embryogenesis: Comparison with retinoic acid receptor mRNA localization

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Abstract
We report results from a study of Hox‐7 expression during mouse embryonic and fetal development and compare the localization of Hox‐7 transcripts with those of the retinoic acid receptors. Transcripts were detected by in situ hybridization. Hox‐7 expression occurs in (1) cephalic neural crest and its derivatives, (2) sites of ectomesodermal interaction, (3) extraembryonic tissues, and (4) endocardial cells. Hox‐7 does not seem to be involved in defining rostrocaudal boundaries, but instead appears to be expressed along the proximodistal axes at these sites. We further investigated the active sites of morphogenesis, which involve an ectomesodermal interaction (e.g., limb buds, visceral arches), including genital tubercle and tail ridge. These are regions highly positive for Hox‐7 transcripts, and many are known to be sites for the expression of γ‐retinoic acid receptors (RARs) and cellular retinoic acid binding proteins. Most regions that express Hox‐7 are subregions of γ‐RAR expression. In the developing limb bud, expression of Hox‐7 takes place in the interdigital region, where it overlaps areas of β‐RAR expression.