Automated Analysis of Feline Platelets in Whole Blood, Including Platelet Count, Mean Platelet Volume, and Activation State

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Abstract
— A new whole‐blood flow cytometric method has been developed for counting and sizing platelets in samples from cats, a species in which platelet and red blood cell sizes overlap significantly. The method is a modified version of the two‐angle laser light scattering technology used by Bayer H*System hematology analyzers. The new method provided accurate platelet counts and mean platelet volumes (MPV, fl) for cats. The method also measured mean platelet component concentration (MFC, g/dl), a parameter which was shown to be sensitive to platelet activation state, and which decreased in value as activation progressed.