Effects of dicyclohexylcarbodi-imide on proton translocation coupled to fumarate reduction in anaerobically grown cells of Escherichia coli K-12

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Abstract
The addition of dicyclohexylcarbodiimide to anaerobic cells of E. coli K12 decreases the observed extent of proton translocation coupled to fumurate reduction by endogenous substrates and the t1/2 [half-life] of proton re-entry after such translocation, but does not affect fumarate uptake. Dicyclohexylcarbodiimide also inhibits fumurate reductase activity in cell extracts.