Intracellular pH topography: determination by a fluorescent probe
- 13 June 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 156 (2) , 227-230
- https://doi.org/10.1016/0014-5793(83)80501-8
Abstract
The distribution of pH inside the yeast Endomyces magnusii was measured at 1 μm resolution using different external pH values. In a neutral buffer the pH of the cytoplasm was 6.7–7.2 in the center, decreasing to 6.0 toward the periphery of the cell. A decrease of external pH was followed by a gradual uniform decrease of internal pH. Using a comparison of the ‘pH map’ with phase-contrast picture of the same cell, the pH of the vacuoles was estimated to be 5.5–5.6.Keywords
This publication has 5 references indexed in Scilit:
- Intracellular pH of yeast cells measured with fluorescent probesFEBS Letters, 1982
- Ion Transport in yeastBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1981
- Measurement of intracellular pHBioscience Reports, 1981
- Intracellular pH.Physiological Reviews, 1981
- Phosphorus-31 nuclear magnetic resonance studies of wild-type and glycolytic pathway mutants of Saccharomyces cerevisiaeBiochemistry, 1979