The identification of three new biosynthetic intermediates and one further biosynthetic enzyme in the clavulanic acid pathway
- 1 January 1993
- journal article
- research article
- Published by Royal Society of Chemistry (RSC) in Journal of the Chemical Society, Chemical Communications
- No. 15,p. 1212-1214
- https://doi.org/10.1039/c39930001212
Abstract
Labelling experiments are described that identify three new compounds, N2-(2-carboxyethyl)arginine, 5-guanidino-(2-oxoazetidin-1-yl)pentanoic acid, and 3-hydroxy-5-guanidino-2-(2-oxoazetidin-1-yl)pentanoic acid as biosynthetic precursors of proclavaminic acid and hence clavulanic acid in Streptomyces clavuligerus TCC 27064 and a new amidino hydrolase, which hydrolyses 3-hydroxy-5-guanidino-2-(2-oxoazetidin-1-yl)pentanoic acid to proclavaminic acid has been characterised.Keywords
This publication has 0 references indexed in Scilit: