Demethylation of trimethylated histone H3 Lys4 in vivo by JARID1 JmjC proteins

18 February 2007

journal article
research article
Published by Springer Nature in Nature Structural & Molecular Biology

Vol. 14 (3) , 240-242
https://doi.org/10.1038/nsmb1200

Abstract

Histone H3 Lys4 trimethylation (H3-K4me3) is a conserved mark of actively transcribed chromatin. Using a conditional mutant of the yeast H3-K4 methyltransferase, Set1p, we demonstrate rapid turnover of H3-K4me3 and H3-K4me2 in vivo and show this process requires Yjr119Cp, of the JARID1 family of JmjC proteins. Ectopic overexpression of mouse Jarid1B, a Yjr119Cp homolog, greatly diminished H3-K4me3 and H3-K4me2 in HeLa cells, suggesting these proteins function as K4 demethylases in vivo.

Keywords

This publication has 18 references indexed in Scilit:

It takes a PHD to interpret histone methylation
Nature Structural & Molecular Biology, 2006
Chromatin Modifications by Methylation and Ubiquitination: Implications in the Regulation of Gene Expression
Annual Review of Biochemistry, 2006
The putative oncogene GASC1 demethylates tri- and dimethylated lysine 9 on histone H3
Nature, 2006
Reversal of Histone Lysine Trimethylation by the JMJD2 Family of Histone Demethylases
Published by Elsevier ,2006
Structural Insights into Histone Demethylation by JMJD2 Family Members
Cell, 2006
Mutations in the JARID1C Gene, Which Is Involved in Transcriptional Regulation and Chromatin Remodeling, Cause X-Linked Mental Retardation
American Journal of Human Genetics, 2005
Histone Demethylation Mediated by the Nuclear Amine Oxidase Homolog LSD1
Cell, 2004
Histone H3 lysine 4 methylation patterns in higher eukaryotic genes
Nature Cell Biology, 2003
Human PLU-1 Has Transcriptional Repression Properties and Interacts with the Developmental Transcription Factors BF-1 and PAX9
Journal of Biological Chemistry, 2003
Targeted Recruitment of Set1 Histone Methylase by Elongating Pol II Provides a Localized Mark and Memory of Recent Transcriptional Activity
Molecular Cell, 2003