Incorporation of radioactive phosphorus into the ribonucleic acid of subfractions derived from guinea-pig-liver microsomes

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Abstract
Guinea pigs were killed at various time intervals after intravenous injection of p32-phosphate. The liver-microsome fraction was sub-fractionated by the method of Simkin and Work, ribonucleic acid of the various subtractions was isolated and specific radioactivity determined. In one experiment, the ribonucleic acids isolated from 3 subtractions were hydrolyzed to the mononucleotides and their specific radioactivities determined. The ribonucleic acid was free of P32-containing contaminants. Incorporation of P32 into the ribonucleic acid of the various microsome subtractions occurred at different rates. The ribonucleoprotein subtraction C had the lowest rate of ribonucleic acid turnover, but the highest rate of protein turnover. These results are discussed in relation to other work on ribonucleic acid turnover during protein synthesis.