Use of Dubos Medium for Culture of M. tuberculosis from Sputum

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Abstract
The use of a mild reagent (2.5-5% soln. of (NH4)2CO3; 40 ml. for 1-3 ml. of sputum) for homogeniza-tion and cone. of tuberculous sputa and the addition of penicillin (0.5-2 U./ml.) to the culture medium (5 ml. in each tube, 25 mm. diam.) permits the successful application of Dubos medium to the routine culture of Mycobacterium tuberculosis from sputum. Of 400 examined sputa, 34 (8.5%) were positive by culture methods and negative by microscopic examination of sputum concentrate (sputum concentrated by treatment with tergitol and Na hypochlorite). The advantage of the culture method is obvious. As compared with other culture methods, the use of Dubos media is inexpensive, easily learned by the technician, suitable for daily examination of a large number of specimens, and allows rapid (within 8-15 days) culture diagnosis of sputa containing relatively small numbers of M. tuberculosis.

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