Annexin II inhibits calcium-dependent phospholipase A1and lysophospholipase but not triacyl glycerol lipase activities of rat liver hepatic lipase
- 16 January 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 296 (3) , 237-240
- https://doi.org/10.1016/0014-5793(92)80294-q
Abstract
A member of the annexin family (the heterotetrameric annexin II2pl 12 complex purified from porcine intestinal epithelium) was tested for its ability to affect different calcium-dependent intrinsic lipolytic activities of rat liver hepatic lipase (HL). Whereas annexin II in the presence of calcium failed to interfere with HL triacyl glycerol lipase (EC 3.1.1.3) activity, it inhibited HL phospholipase A1 (EC 3.1.1.32) and lysophospholipase (EC 3.1.1.5) activities. Inhibition could be overcome by increasing the substrate concentration. Under phospholipase A1 assay conditions, annexin II did not bind to the purified HL enzyme. These results therefore suggest that only inhibitor/substrate interactions lead to inhibition of HL phospholipase A1 and lysophospholipase activities, an obviously general mechanism of phospholipase inhibition by annexins. Possible implications of HL inhibition in vivo by annexins are discussed.Keywords
This publication has 35 references indexed in Scilit:
- Kinetic evidence for phosphatidylethanolamine and triacylglycerol as preferential substrates for hepatic lipase in HDL subfractions: Modulation by changes in the particle surface, or in the lipid coreBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- Lipocortin I and lipocortin II inhibit phosphoinositide‐ and polyphosphoinositide‐specific phospholipase C The effect results from interaction with the substratesFEBS Letters, 1989
- Phosphatidylcholine and triacylglycerol hydrolysis in HDL as induced by hepatic lipase: modulation of the phospholipase activity by changes in the particle surface or in the lipid coreBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1989
- Dexamethasone-induced reduction of phospholipase D activity in the rat Possible role of lipocortinFEBS Letters, 1988
- Lipocortin inhibition of extracellular and intracellular phospholipases A2 is substrate concentration dependentFEBS Letters, 1987
- Lipoprotein lipases and stress hormones: studies with glucocorticoids and cholera toxinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1986
- Hepatic lipase purification and characterizationBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1984
- Regulation of liver lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- The effect of corticotrophin on liver-type lipase activity in adrenals, liver and high-density lipoprotein subfractions in the ratBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1983
- Triacylglycerol lipase, monoacylglycerol lipase and phospholipase activities of highly purified rat hepatic lipaseBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1982