Normal murine melanocytes in culture
- 1 July 1987
- journal article
- rapid communications-in-cell-biology
- Published by Springer Nature in In Vitro Cellular & Developmental Biology
- Vol. 23 (7) , 519-522
- https://doi.org/10.1007/bf02628423
Abstract
Summary A major obstacle to applying the techniques of molecular biology to the genetics and cell biology of pigmentation has been our inability to grow normal murine melanocytes in culture. We report here the establishment and characterization of continuously proliferating cultures of cutaneous pigment cells from seven strains of mice. Melanocytes were grown from the dermis of newborn mice in medium containing 12-0-tetradecanoyl-13-phorbol-acetate; a substance, such as melanotropin, that raises intracellular levels of cyclic AMP; and an extract made from human placenta.Keywords
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