The production of α-hydroxyglutaric acid from glutamic acid by cell-free preparations of Peptococcus aerogenes

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Abstract
Active cell-free extracts of Peptococcus aerogenes were prepared which metabolized glutamic acid to α-hydroxyglutaric acid. Factors affecting the formation of this intermediate were studied by following the conversion of glutamic acid labelled with 14C in the one or five positions. The results of these experiments revealed that the production of α-hydroxyglutaric acid from glutamic acid by cell-free extracts was NAD-dependent. The labelled α-hydroxyglutaric acid produced by NAD-supplemented extracts was purified by anion exchange chromatography and identified by several methods including paper and thin-layer chromatography, mass spectrometry, and infrared spectroscopy. A pathway has been proposed for the conversion of glutamic acid to α-hydroxyglutaric acid by cell-free extracts of P. aerogenes.

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